Research


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Research project (§ 26 & § 27)
Duration : 2018-08-01 - 2020-01-31

The enzyme cellbiose dehydrogenase is a fungal enzyme containing flavin and heme. The catalytically active dehydrogenase domain catalyses the oxidation of cellobiose to cellobionic acid and lactose to lactobionic acid. The enzyme is a dehydrogenase and only reacts poorly with oxygen. A cellobiose oxidase showing improved activity with oxygen would have advantages with respect to various applications. Generating a CDH variant of Myriococcum thermophilum with increased reactivity with oxygen, the neutral pH activity optimum was maintained. It was shown that this variant can convert lactose in milk. It is the aim of the current project to further improve the enzyme for industrial applications.
Research project (§ 26 & § 27)
Duration : 2018-08-01 - 2021-07-31

Modern medical diagnostics is moving towards continuous measurements, which provide data in real time. An example for this can be biosensors that are implanted in the skin and measure blood glucose levels. Currently, stability of enzyme-based sensors is limited by several days, yet very stable enzymes could extend this to several weeks or even months. Cellobiose dehydrogenase (CDH) is a redox enzyme that based on its uniques architecture enables direct electron transfer to electrodes. Asa flavocyctochrome, CDH is a very stable enzyme, and its inactivation mechanisms are not explored to date. It could be shown though that hydrogen peroxide, which can be fored as a byproduct, results in inactivation, similar to glucose oxidase, which is the most frequently ezyme in blood glucose sensors. The project STARELIS will elucidate the inactivation menchanisms relevant for CDH and thus should serve as the basis for the development of stable, implantable glucose sensors. By combining various biochemical and biophysical analytical methods together with enzyme engineering and molecular modeling the project is aiming at elucidating the effect of oxidative stress on CDH.
Research project (§ 26 & § 27)
Duration : 2018-06-01 - 2020-05-31

Aim of the project is the monitoring and improvement of the production hygiene with a main focus on building services engineering and ventilation. This also includes aspects such as cleaning and decontamination as well as the development of new analytical methods. Based on the evaluation of current manufacturing practices in the Life Science Industries, experimental and simulation tools will be applied in order to investigate distribution pathways of contaminants. Subsequently, effective decontamination strategies will be established based on cleaning and decontamination, the application of antimicrobial surfaces as well as the application of ventilation and cleanroom concepts and the hygienic design of equipment. Finally, recommendations and guidelines will be developed in order to support the Life Science Industries in improving their production efficiency and product safety by optimized hygiene monitoring, cleaning and decontamination as well as aseptic processing concepts.

Supervised Theses and Dissertations