Research


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Research project (§ 26 & § 27)
Duration : 2017-03-01 - 2022-02-28

The mission of OXIDISE is to resolve authentic enzymatic activities of lignocellulose degrading oxidoreductases when bound onto their polymeric substrates and to elucidate their interaction. To this purpose, high-resolution techniques will be developed in the project. Fungal oxidoreductases involved in lignocellulose processing attracted great attention in the last years - like the discovery of oxidative cellulose degradation by lytic polysaccharide monooxygenase (LPMO). Over 90% of biomass degrading fungal genomes contain oxidoreductases (LPMO, cellobiose dehydrogenase, laccase, lignin peroxidase, aryl alcohol oxidase,…) involved in the oxidative cleavage of the recalcitrant biopolymers cellulose, hemicellulose or lignin. The elucidation of these enzyme mechanisms, interactions and kinetics is the key to understand fungal physiology and optimise biomass saccharification and biorefineries. To circumvent typical problems associated with heterogeneous reactions, assaying techniques should have a high spatial and temporal resolution. OXIDISE will develop and apply techniques based on microelectrodes, scanning electron microscopy (SECM), surface-enhanced raman scattering (SERS) and microscopic fluorescence techniques to pursue five objectives: to 1) develop enzyme-modified electrodes for the detection of lignocellulose oxidising enzymes or their products. 2) miniaturise and assemble microelectrode arrays with a high spatial resolution. 3) transfer microelectrode modifications to SECM for increased spatial resolution (~10 nm) and scanning of areas. 4) investigate the interaction of oxidoreductases on polymeric substrates, e.g. the interaction of CDH/LPMO or more complex enzyme systems. 5) transfer the developed techniques to wood samples and growing fungal hyphae and their secretome. OXIDISE takes a new approach to provide crucial insight into the function of important enzymes, which so far has been somewhat neglected, possibly because of the involved experimental challenges.
Research project (§ 26 & § 27)
Duration : 2017-08-01 - 2017-12-31

Within the scope of the described project, antibiotic resistance genes are detected and identified on plasmids, isolated from Escherichia coli transconjugants by means of a specific microarray-based technique. The plasmids to be investigated were obtained in the context of a previous project by the application of in situ mating with a specific Escherichia coli recipient strain directly from samples, which are assigned to the animal production chain. The applied microarray technique was tailored in a previous research work.
Research project (§ 26 & § 27)
Duration : 2017-01-01 - 2017-12-31

Within the project the microbiota of selected sourdoughs will be analysed in detail. Next to the molecular monitoring of the microecological dynamic of the sourdoughs lactic acid bacteria and yeasts will be isolated, characterised and stored in a strain bank. The aim of the collaboration with the project partner Versuchsanstalt für Getreideverarbeitung (VG) is to gain the knowledge about the formulation of sourdoughs for the production of high quality bread. Together with the partner an Austrian sourdough database will be set up that will be used by the project partner to help Austrian backeries to improve the quality of their products.

Supervised Theses and Dissertations