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Research project (§ 26 & § 27)
Duration : 2018-08-01 - 2020-01-31

The enzyme cellbiose dehydrogenase is a fungal enzyme containing flavin and heme. The catalytically active dehydrogenase domain catalyses the oxidation of cellobiose to cellobionic acid and lactose to lactobionic acid. The enzyme is a dehydrogenase and only reacts poorly with oxygen. A cellobiose oxidase showing improved activity with oxygen would have advantages with respect to various applications. Generating a CDH variant of Myriococcum thermophilum with increased reactivity with oxygen, the neutral pH activity optimum was maintained. It was shown that this variant can convert lactose in milk. It is the aim of the current project to further improve the enzyme for industrial applications.
Research project (§ 26 & § 27)
Duration : 2018-09-01 - 2019-08-31

In this project, the E. coli bacterial counts are enumerated in samples from an externally performed feeding trial. The determination of the germ count takes place without selection pressure as well as with specially adapted antibiotics containing media. Subsequently, defined numbers of presumptive E. coli isolates per sample are obtained, purified and identified by MALDI-TOF MS at the species level. These isolates are then tested for antibiotic susceptibility based on current international guidelines.
Research project (§ 26 & § 27)
Duration : 2018-08-01 - 2021-07-31

Modern medical diagnostics is moving towards continuous measurements, which provide data in real time. An example for this can be biosensors that are implanted in the skin and measure blood glucose levels. Currently, stability of enzyme-based sensors is limited by several days, yet very stable enzymes could extend this to several weeks or even months. Cellobiose dehydrogenase (CDH) is a redox enzyme that based on its uniques architecture enables direct electron transfer to electrodes. Asa flavocyctochrome, CDH is a very stable enzyme, and its inactivation mechanisms are not explored to date. It could be shown though that hydrogen peroxide, which can be fored as a byproduct, results in inactivation, similar to glucose oxidase, which is the most frequently ezyme in blood glucose sensors. The project STARELIS will elucidate the inactivation menchanisms relevant for CDH and thus should serve as the basis for the development of stable, implantable glucose sensors. By combining various biochemical and biophysical analytical methods together with enzyme engineering and molecular modeling the project is aiming at elucidating the effect of oxidative stress on CDH.

Supervised Theses and Dissertations